FAM49B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM49B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20127-1-AP

Synonyme

BM 009, FAM49B, L1, Protein FAM49B

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 20127-1-AP

  WB result of FAM49B antibody (20127-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM49B transfected MCF-7 cells.

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  WB analysis of mouse testis using 20127-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20127-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 20127-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20127-1-AP

  IP Result of anti-FAM49B (IP:20127-1-AP, 4ug; Detection:20127-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20127-1-AP

  Immunofluorescent analysis of HepG2 cells, using FAM49B antibody 20127-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hodengewebe, humanes Plazenta-Gewebe, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below

Produktinformation

20127-1-AP bindet in WB, IP, IHC, IF, ELISA FAM49B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM49B fusion protein Ag13939
• Vollständiger Name: family with sequence similarity 49, member B
• Berechnetes Molekulargewicht: 324 aa, 37 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC003599
• Gene symbol: FAM49B
• Gene ID (NCBI): 51571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
monkey	WB	Nat Cell Biol; Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.; Authors - Loic Fort ; KD Validated
human	WB	Genes (Basel); Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.; Authors - Yijian Chen ; KO Validated
mouse	WB	Mol Immunol; FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.; Authors - Zuotian Huang ; KD Validated