FAM20C Polyklonaler Antikörper

FAM20C Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 25395-1-AP

Synonyme

Dentin matrix protein 4, DMP 4, DMP4, FAM20C, Protein FAM20C, RNS

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 25395-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25395-1-AP (FAM20C Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

25395-1-AP bindet in WB, IHC, ELISA FAM20C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM20C fusion protein Ag22061
• Vollständiger Name: family with sequence similarity 20, member C
• Berechnetes Molekulargewicht: 584 aa, 66 kDa
• GenBank-Zugangsnummer: BC040074
• Gene symbol: FAM20C
• Gene ID (NCBI): 56975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM20C, also named as Golgi-enriched fraction casein kinase or Dentin matrix protein 4, is a 584 amino acid protein, which belongs to the FAM20 family. FAM20C is widely expressed. FAM20C as a Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP).

Publikationen

Species	Application	Title
monkey	WB	Nat Commun; Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia; Authors - Toshiyasu Koike
rat	WB,IHC	FASEB J; Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis.; Authors - Elena Tibaldi
mouse	WB	JCI Insight; 1,25-Dihydroxyvitamin D3 regulates furin-mediated FGF23 cleavage; Authors - Han Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Doug (Verified Customer) (05-01-2019)

Does not work. This antibody is garbage. Don't waste your money.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: MDA-MB231 a line that I can readily detect FAM20C by MS

FAM20C Antibody Western Blot, validation (1/1000 dilution) in MDA-MB231 a line that I can readily detect FAM20C by MS (Cat no:25395-1-AP)