FAM134B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM134B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 21537-1-AP

Synonyme

FAM134B, JK-1, Protein FAM134B, Reticulophagy receptor 1, RETREG1

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 21537-1-AP

  WB result of FAM134B antibody (21537-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM134B transfected HEK-293 cells.

• 

  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HEK-293-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 29 publications below
• IF: See 8 publications below

Produktinformation

21537-1-AP bindet in WB, IHC, IF, ELISA FAM134B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM134B fusion protein Ag16176
• Vollständiger Name: family with sequence similarity 134, member B
• Berechnetes Molekulargewicht: 497 aa, 55 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC030517
• Gene symbol: FAM134B
• Gene ID (NCBI): 54463
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham ; KD Validated
human	WB	Nat Commun; Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.; Authors - Mark L Schultz
mouse	WB,IF	Dev Cell; Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development; Authors - Patrick Lüningschrör
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	WB	EMBO J; CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.; Authors - Thaddaeus Mutugi Nthiga
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

H (Verified Customer) (04-06-2020)

The antibody works well. There were a few non-specific bands in HEK293T lysates though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FAM134B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21537-1-AP)