FAM103A1 Polyklonaler Antikörper

FAM103A1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19422-1-AP

Synonyme

C15orf18, FAM103A1, HsT19360, Protein FAM103A1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 19422-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19422-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 19422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19422-1-AP (FAM103A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

19422-1-AP bindet in WB, IHC, IF, ELISA FAM103A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM103A1 fusion protein Ag13749
• Vollständiger Name: family with sequence similarity 103, member A1
• Berechnetes Molekulargewicht: 118 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC003627
• Gene symbol: FAM103A1
• Gene ID (NCBI): 83640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM103A1, also named as RNMT-activating mini protein, is a 118 amino acid protein, which belongs to the RAM family. FAM103A1 as a component of mRNA cap binding complex localizes in the nucleus. FAM103A1 is required for efficient mRNA cap methylation and regulates RNMT expression by a post-transcriptional stabilizing mechanism.

Publikationen

Species	Application	Title
human	WB	J Mol Biol; Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.; Authors - Michael J Osborne