FAK Monoklonaler Antikörper

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• KD/KO Validated

FAK Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2C5B9

Kat-Nr. : CL555-66258

Synonyme

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Galerie der Validierungsdaten

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  IF Staining of A549 using CL555-66258

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200.

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  IF Staining of HUVEC using CL555-66258

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of A549 using CL555-66258

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A549-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66258 bindet in IF FAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FAK fusion protein Ag17966
• Vollständiger Name: PTK2 protein tyrosine kinase 2
• Berechnetes Molekulargewicht: 1052 aa, 119 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC028733
• Gene symbol: PTK2
• Gene ID (NCBI): 5747
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.