FADD Polyklonaler Antikörper

FADD Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Rezensionen (1)

Kat-Nr. : 14906-1-AP

Synonyme

FADD, GIG3, MORT1, Protein FADD

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Galerie der Validierungsdaten

• 

  WB analysis using 14906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14906-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14906-1-AP

  mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 14906-1-AP

  IP Result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug.

• 

  IHC staining of human lung cancer using 14906-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14906-1-AP (FADD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 14906-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14906-1-AP (FADD antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HT-1080 using 14906-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HT-1080 cells using 14906-1-AP (FADD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HT-1080.Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 31 publications below
• IHC: See 4 publications below
• IP: See 3 publications below

Produktinformation

14906-1-AP bindet in WB, IP, IHC, IF, ELISA FADD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FADD fusion protein Ag6701
• Vollständiger Name: Fas (TNFRSF6)-associated via death domain
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC000334
• Gene symbol: FADD
• Gene ID (NCBI): 8772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Publikationen

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	WB	PLoS Biol; A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.; Authors - Yingying Zhang
human	WB	Eur J Med Chem; PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism; Authors - Hongyan Lin
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
rat	WB	Cell Cycle; N-acetylserotonin protects PC12 cells from hydrogen peroxide induced damage through ROS mediated PI3K / AKT pathway.; Authors - Jihe Kang
human	IP, WB	Front Immunol; Crosstalk Between NDP52 and LUBAC in Innate Immune Responses, Cell Death, and Xenophagy.; Authors - Hirohisa Miyashita

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Isha (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:800 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: kidney

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