FABP7-Specific Polyklonaler Antikörper

FABP7-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14836-1-AP

Synonyme

B FABP, BLBP, Brain lipid binding protein, FABP7, FABP7-Specific, FABPB, Fatty acid binding protein 7, MRG

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14836-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 14836-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14836-1-AP bindet in WB, IHC, IF, ELISA FABP7-Specific und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fatty acid binding protein 7, brain
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14-15 kDa
• GenBank-Zugangsnummer: NM_001446
• Gene symbol: FABP7
• Gene ID (NCBI): 2173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP7 (Fatty Acid Binding Protein-7), also known as B-FABP (Brain Fatty Acid-Binding Protein), is a member of FABP family which are intracellular proteins involved in lipid metabolism. Normally FABP7 is mainly expressed in brain and other neural tissues and is important in early nervous system development. In addition, FABP7 is considered a marker for some brain tumors and may have a role in tumorigenesis in the peripheral nervous system and in several extra nervous system neoplasms such as breast cancer, renal cancer, and melanoma. This antibody specially recognizes FABP7 and not cross-react with other FABPs.

Publikationen

Species	Application	Title
	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu