FABP3 Monoklonaler Antikörper

FABP3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2B5C1

Kat-Nr. : 60280-1-Ig

Synonyme

FABP11, FABP3, Fatty acid binding protein 3, H FABP, M FABP, MDGI, O FABP

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Galerie der Validierungsdaten

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  WB analysis using 60280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60280-1-Ig (FABP3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 60280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, rat heart, mouse heart
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1900-1:7600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

60280-1-Ig bindet in WB, IHC, IF, ELISA FABP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FABP3 fusion protein Ag21483
• Vollständiger Name: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC007021
• Gene symbol: FABP3
• Gene ID (NCBI): 2170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

Publikationen

Species	Application	Title
human	IF	J Cell Mol Med; Four transcription profile-based models identify novel prognostic signatures in oesophageal cancer.; Authors - Tongyan Liu
human	WB	Mol Cell Proteomics; Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway; Authors - Victor Laigle
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu