FA2H Polyklonaler Antikörper

FA2H Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15452-1-AP

Synonyme

FA2H, FAAH, FAH1, fatty acid 2 hydroxylase, Fatty acid alpha hydroxylase, FAXDC1, SCS7

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Galerie der Validierungsdaten

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  WB analysis using 15452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 15452-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15452-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15452-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15452-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15452-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15452-1-AP (FA2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Magengewebe, Maushirngewebe, Maus-Kolongewebe, MKN-45-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

15452-1-AP bindet in WB, IHC, IF, ELISA FA2H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FA2H fusion protein Ag7717
• Vollständiger Name: fatty acid 2-hydroxylase
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: BC002679
• Gene symbol: FA2H
• Gene ID (NCBI): 79152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Cell Lineage-Based Stratification for Glioblastoma.; Authors - Zilai Wang
		Dev Cell; Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy.; Authors - Xuanhua P Xie
human	WB,IHC	Front Oncol; FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control.; Authors - Xiaofeng Dai
human	WB	J Med Chem; Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.; Authors - Tyler D Baguley
human	WB	Biochem Biophys Res Commun; Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration.; Authors - Masayo Hirao-Suzuki
human	WB	J Toxicol Sci; Perfluorooctanoic acid (PFOA) as a stimulator of estrogen receptor-negative breast cancer MDA-MB-231 cell aggressiveness: Evidence for involvement of fatty acid 2-hydroxylase (FA2H) in the stimulated cell migration.; Authors - Genki Sakai