Factor XII Monoklonaler Antikörper

Factor XII Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2E2F11

Kat-Nr. : 66089-1-Ig

Synonyme

Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 66089-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66089-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66089-1-Ig(Factor XII antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 66089-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66089-1-Ig

  Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K562-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslebergewebe, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66089-1-Ig bindet in WB, IHC, IF,ELISA Factor XII und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Factor XII fusion protein Ag3401
• Vollständiger Name: coagulation factor XII (Hageman factor)
• Berechnetes Molekulargewicht: 615 aa, 68 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC012390
• Gene symbol: F12
• Gene ID (NCBI): 2161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Publikationen

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.; Authors - Fanfan Li