Endoglin/CD105 Monoklonaler Antikörper

Endoglin/CD105 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2D5E8

Kat-Nr. : CL555-67075

Synonyme

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

---

Galerie der Validierungsdaten

• 

  IF Staining of human tonsillitis using CL555-67075

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Endoglin/CD105 antibody (CL555-67075, Clone: 2D5E8 ) at dilution of 1:600.

• 

  IF Staining of human tonsillitis using CL555-67075

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Endoglin/CD105 antibody (CL555-67075, Clone: 2D5E8 ) at dilution of 1:600.

• 

  IF Staining of human breast cancer using CL555-67075

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Endoglin/CD105 antibody (CL555-67075, Clone: 2D5E8 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-67075

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Endoglin/CD105 antibody (CL555-67075, Clone: 2D5E8 ) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-67075 bindet in IF Endoglin/CD105 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Ag27837
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: ENG
• Gene ID (NCBI): 2022
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)