EXOSC4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOSC4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 15937-1-AP

Synonyme

EXOSC4, exosome component 4, hRrp41p, p12A, RRP41, RRP41A, Rrp41p, SKI6, Ski6p

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Galerie der Validierungsdaten

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  WB analysis using 15937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15937-1-AP (EXOSC4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15937-1-AP

  WB result of EXOSC4 antibody (15937-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC4 transfected Jurkat cells.

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HCT 116-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

15937-1-AP bindet in WB, IHC, IF, ELISA EXOSC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC4 fusion protein Ag7595
• Vollständiger Name: exosome component 4
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC002777
• Gene symbol: EXOSC4
• Gene ID (NCBI): 54512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. However, studies have shown that EXOSC4 can interact with EXOSC2 and EXOSC7, possibly form a complex with the molecular weight of 90 kDa (PMID: 25115889). Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).

Publikationen

Species	Application	Title
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
human	WB,IHC	Life Sci; STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.; Authors - Yong-Xia Wang ; KD Validated
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (07-06-2022)

Control and Exosc4 depletion shows a reduction of Exosc4 signal. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC4 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:15937-1-AP)