EXOSC2 Monoklonaler Antikörper

EXOSC2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G8B1

Rezensionen (2)

Kat-Nr. : 66099-1-Ig

Synonyme

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

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Galerie der Validierungsdaten

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  WB analysis using 66099-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HeLa using 66099-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66099-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66099-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin cancer using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin cancer using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 66099-1-Ig

  Immunofluorescent analysis of () fixed HepG2 cells using 66099-1-Ig (EXOSC2 antibody) at dilution of 1:25.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

66099-1-Ig bindet in WB, IHC, IF, ELISA EXOSC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EXOSC2 fusion protein Ag7003
• Vollständiger Name: exosome component 2
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000747
• Gene symbol: EXOSC2
• Gene ID (NCBI): 23404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Publikationen

Species	Application	Title
human		Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel
human	WB	Front Endocrinol (Lausanne); FGF2 Is Protective Towards Cisplatin-Induced KGN Cell Toxicity by Promoting FTO Expression and Autophagy.; Authors - Rongli Wang
human	WB	Reprod Biol Endocrinol; FTO protects human granulosa cells from chemotherapy-induced cytotoxicity.; Authors - Rongli Wang
human	WB	bioRxiv; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication.; Authors - Tobias Moll
human	WB	J Oncol; Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway; Authors - Meng Wang
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikolaus (Verified Customer) (07-14-2022)

Exosc2 depletion is used as a control. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:66099-1-Ig)

FH

Tsimafei (Verified Customer) (06-01-2022)

WT and Exosc2 depletion were compared. There is a clear reduction of Exosc2 signal upon the depletion. Though, the strength of the signla isn't great. Antibodies were incubated at 4C, ON.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:66099-1-Ig)