SEC6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SEC6 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14703-1-AP

Synonyme

EXOC3, exocyst complex component 3, Exocyst complex component Sec6, SEC6, SEC6L1, Sec6p

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14703-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14703-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14703-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14703-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14703-1-AP

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

14703-1-AP bindet in WB, IHC, IF, ELISA SEC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC6 fusion protein Ag6383
• Vollständiger Name: exocyst complex component 3
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC064569
• Gene symbol: EXOC3
• Gene ID (NCBI): 11336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Microbiol; Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.; Authors - Hiroki Takeuchi ; KD Validated
human	WB	Cell Microbiol; Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.; Authors - Takeuchi Hiroki H ; KD Validated
rat	WB	Dev Biol; Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition.; Authors - Youssef G G