EXO1 Monoklonaler Antikörper

EXO1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11D1

Kat-Nr. : 68536-1-Ig

Synonyme

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

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Galerie der Validierungsdaten

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  WB analysis using 68536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68536-1-Ig (EXO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293T-Zellen, HeLa-Zellen, K-562-Zellen, MOLT-4-Zellen, TF-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68536-1-Ig bindet in WB, ELISA EXO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EXO1 fusion protein Ag9253
• Vollständiger Name: exonuclease 1
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC007491
• Gene symbol: EXO1
• Gene ID (NCBI): 9156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.