EXO1 Polyklonaler Antikörper

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• KD/KO Validated

EXO1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16253-1-AP

Synonyme

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

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Galerie der Validierungsdaten

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  WB analysis using 16253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16253-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 16253-1-AP

  IP Result of anti-EXO1 (IP:16253-1-AP, 4ug; Detection:16253-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche IP: Maus-Thymusgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below

Produktinformation

16253-1-AP bindet in WB, IP, ELISA EXO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXO1 fusion protein Ag9253
• Vollständiger Name: exonuclease 1
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC007491
• Gene symbol: EXO1
• Gene ID (NCBI): 9156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.; Authors - Margaret Tammaro
human	WB	Nucleic Acids Res; PCNA promotes processive DNA end resection by Exo1.; Authors - Chen Xiaoqing X ; KD Validated
human	WB	Elife; Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations.; Authors - Birong Shen ; KD Validated
human	WB	PLoS Genet; EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.; Authors - Yuehan Wu
rat	WB	J Cardiovasc Transl Res; Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.; Authors - Xinliang Zou
human	WB	Front Genet; Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.; Authors - Christopher B Nelson ; KD Validated