EXD2 Polyklonaler Antikörper

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• KD/KO Validated

EXD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20138-1-AP

Synonyme

3'-5' exoribonuclease EXD2, C14orf114, EC:3.1.11.1, EC:3.1.13.-, EXDL2

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  WB analysis of mouse lung using 20138-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 20138-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 20138-1-AP

  IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.

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  IHC staining of human lung cancer using 20138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20138-1-AP (EXD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20138-1-AP (EXD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20138-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Maushirngewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20138-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EXD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXD2 fusion protein Ag14090
• Vollständiger Name: exonuclease 3'-5' domain containing 2
• Berechnetes Molekulargewicht: 70 kD aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC001962
• Gene symbol: EXD2
• Gene ID (NCBI): 55218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXD2 (Exonuclease 3'-5' Domain Containing 2), is a protein with exonuclease activity of 3'-5' nucleic acid, which is involved in many biological processes in cells, including DNA repair, RNA processing and mitochondrial translation. EXD2 protein is mainly located in mitochondrial outer membrane, intermediate fiber cytoskeleton and DNA damage site. This protein may be a potential drug target in some cancers. For example, in ALT (telomere selective elongation) dependent cancer, the co-elimination of EXD2 with BLM, DNA2 or POLD3 may mediate synthetic lethality. In addition, EXD2 inhibitors may exert anticancer effects by inhibiting mitochondrial translation and metabolic reprogramming. It has two isoforms with molecular weights of 56 kDa and 70 kDa respectively.

Publikationen

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.; Authors - Xikui Sun ; KO Validated
mouse	WB,IF	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu