EWS Polyklonaler Antikörper

EWS Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 55191-1-AP

Synonyme

EWS, EWS oncogene, EWSR1, RNA binding protein EWS

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Galerie der Validierungsdaten

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  WB analysis using 55191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55191-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55191-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 55191-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

55191-1-AP bindet in WB, IHC, ELISA EWS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Ewing sarcoma breakpoint region 1
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: NM_005243
• Gene symbol: EWSR1
• Gene ID (NCBI): 2130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Nucleic Acids Res; The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.; Authors - Huiwu Ouyang
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Manohar (Verified Customer) (12-18-2021)

Overnight primary antibody using milk to block the blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293