ESYT1 Monoklonaler Antikörper

ESYT1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2C12E4

Rezensionen (1)

Kat-Nr. : 67688-1-Ig

Synonyme

E Syt1, ESYT1, E-Syt1, Extended synaptotagmin 1, Extended synaptotagmin-1, FAM62A, KIAA0747, MBC2

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Galerie der Validierungsdaten

• 

  WB analysis using 67688-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67688-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

67688-1-Ig bindet in WB, ELISA ESYT1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ESYT1 fusion protein Ag16840
• Vollständiger Name: family with sequence similarity 62 (C2 domain containing), member A
• Berechnetes Molekulargewicht: 1104 aa, 123 kDa
• Beobachtetes Molekulargewicht: 123 kDa
• GenBank-Zugangsnummer: BC004998
• Gene symbol: FAM62A
• Gene ID (NCBI): 23344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair; Authors - Arsila P. K. Ashraf ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shihab (Verified Customer) (09-17-2021)

This antibody was used in a western blot application to identify recombinant Esyt1 prescence. Using 1:500 at the first attempt, this antibody was able to successfully provide a band at the correct size. Furthermore, the band was clean which was an added bonus. I will be reducing the concentration of antibody used and also trying this antibody in other applications as well as primary cell lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 (will be reducing)
• Cell Tissue Type: HEK293 transfected with Esyt1