ESR2 Beta 6 Polyklonaler Antikörper

ESR2 Beta 6 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55471-1-AP

Synonyme

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 6, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 6 antibody (55471-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red), DAPI (Blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Zervixgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55471-1-AP bindet in WB, IHC, IF,ELISA ESR2 Beta 6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: estrogen receptor 2 (ER beta)
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001437
• Gene symbol: ESR2
• Gene ID (NCBI): 2100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual