TXNDC4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TXNDC4 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16016-1-AP

Synonyme

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

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Galerie der Validierungsdaten

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  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16016-1-AP

  WB result of TXNDC4 antibody (16016-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC4 transfected HepG2 cells.

• 

  WB analysis of K-562 using 16016-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16016-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16016-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16016-1-AP

  IP Result of anti-TXNDC4 (IP:16016-1-AP, 4ug; Detection:16016-1-AP 1:500) with K-562 cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16016-1-AP

  Immunofluorescent analysis of Hela cells, using ERP44 antibody 16016-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16016-1-AP bindet in WB, IP, IHC, IF, CoIP TXNDC4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC4 fusion protein Ag8818
• Vollständiger Name: endoplasmic reticulum protein 44
• Berechnetes Molekulargewicht: 406 aa, 47 kDa
• Beobachtetes Molekulargewicht: 44-47 kDa
• GenBank-Zugangsnummer: BC005374
• Gene symbol: ERP44
• Gene ID (NCBI): 23071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.

Publikationen

Species	Application	Title
human	IF	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
human	WB,IF	Antioxid Redox Signal; Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM).; Authors - Anelli Tiziana T
human	WB,IHC,CoIP	J Transl Med; ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.; Authors - Hui Tian ; KD Validated
human	WB	Traffic; Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells.; Authors - Tiziana Anelli
human,mouse	WB,IHC	BMC Cancer; Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma; Authors - Tian Xia