ERK1/2 Monoklonaler Antikörper

ERK1/2 Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

53

CloneNo.

1A3D3

Rezensionen (1)

Kat-Nr. : 67170-1-Ig

Synonyme

ERK, ERK 1, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Galerie der Validierungsdaten

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  WB analysis of ROS1728 using 67170-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67170-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67170-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67170-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 52 publications below
• IHC: See 1 publications below

Produktinformation

67170-1-Ig bindet in WB, IHC, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 3
• Berechnetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: NM_002746
• Gene symbol: MAPK3
• Gene ID (NCBI): 5595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publikationen

Species	Application	Title
rat	WB	J Hazard Mater; ERK/p38/ROS burst responses to environmentally relevant concentrations of diphenyl phosphate-evoked neutrophil extracellular traps formation: Assessing the role of autophagy.; Authors - Siwen Li
mouse	WB	J Hazard Mater; Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.; Authors - Tianxin Zhao
mouse	WB	Cancer Lett; SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.; Authors - Tian-Jiao Li
human	WB	Int J Mol Sci; LMNA Reduced Acquired Resistance to Erlotinib in NSCLC by Reversing the Epithelial–Mesenchymal Transition via the FGFR/MAPK/c-fos Signaling Pathway; Authors - Chunsheng Hu
mouse	WB	J Neuroinflammation; Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia.; Authors - Anan Xu
mouse	WB	Aging (Albany NY); METTL3 regulates hippocampal gene transcription via N6-methyladenosine methylation in sevoflurane-induced postoperative cognitive dysfunction mouse; Authors - Baiqing He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (05-18-2020)

Excellent Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

ERK1/2 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:67170-1-Ig)