ERGIC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERGIC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16108-1-AP

Synonyme

ERGIC 32, ERGIC1, ERGIC32, KIAA1181, NET24

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 16108-1-AP

  WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 16108-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16108-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16108-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16108-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16108-1-AP

  IP Result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.

• 

  IHC staining of human liver cancer using 16108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16108-1-AP

  Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

16108-1-AP bindet in WB, IP, IHC, IF, ELISA ERGIC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERGIC1 fusion protein Ag9025
• Vollständiger Name: endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC012766
• Gene symbol: ERGIC1
• Gene ID (NCBI): 57222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522.; Authors - Shen Mynn Tan
human	WB	Cell Rep; Proteomic Profiling of Mammalian COPII and COPI Vesicles.; Authors - Frank Adolf
human	WB,IF	Nat Commun; Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2; Authors - Karsten Nalbach