ERAP2 Monoklonaler Antikörper

ERAP2 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2B11A2

Kat-Nr. : 67477-1-Ig

Synonyme

ERAP2, FLJ23633, FLJ23701, FLJ23807, L RAP, LRAP

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Galerie der Validierungsdaten

• 

  WB analysis of A549 using 67477-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67477-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67477-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67477-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67477-1-Ig bindet in WB, ELISA ERAP2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERAP2 fusion protein Ag30061
• Vollständiger Name: endoplasmic reticulum aminopeptidase 2
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC065240
• Gene symbol: ERAP2
• Gene ID (NCBI): 64167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase). It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. ERAP2 can form heterodimers with ERAP1(PMID:15908954). Defects in the expression of ERAP2 may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance. ERAP2 has the calculated molecular mass of 105-110 kDa, and apparent molecular mass of 120 kDa duo to the glycosylation.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); ERAP2 as a potential biomarker for predicting gemcitabine response in patients with pancreatic cancer; Authors - Pian Yu