ER Polyklonaler Antikörper

ER Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 20698-1-AP

Synonyme

ER, ER alpha, Era, ERα, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1

---

Galerie der Validierungsdaten

• 

  WB analysis using 20698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20698-1-AP (ER antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 20698-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 20698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (20698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20698-1-AP bindet in WB, IHC, IF, ELISA ER und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: estrogen receptor 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_000125
• Gene symbol: ESR1
• Gene ID (NCBI): 2099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Shinford. Z (Verified Customer) (01-31-2024)

Significant bands were observed across numerous cell lines, including instances where cells were not expected to exhibit such robust expression intensity. It raises the possibility that a monoclonal antibody might yield more precise results compared to the polyclonal antibody currently employed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

ER Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:20698-1-AP)