EPO Polyklonaler Antikörper

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• KD/KO Validated

EPO Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17908-1-AP

Synonyme

EP, EPO, Epoetin, erythropoietin, MVCD2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 17908-1-AP

  WB result of EPO antibody (17908-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPO transfected HEK-293 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17908-1-AP bindet in WB, IHC, ELISA EPO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPO fusion protein Ag12302
• Vollständiger Name: erythropoietin
• Berechnetes Molekulargewicht: 193 aa, 21 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC093628
• Gene symbol: EPO
• Gene ID (NCBI): 2056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Publikationen

Species	Application	Title
human	IHC	Front Genet; Identification and Validation of a Tumor Microenvironment-Related Gene Signature in Hepatocellular Carcinoma Prognosis.; Authors - Changjing Huang
human	WB	Front Mol Neurosci; The cytokine receptor CRLF3 is a human neuroprotective EV-3 (Epo) receptor; Authors - Debbra Y Knorr