EPHX1 Monoklonaler Antikörper

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• KD/KO Validated

EPHX1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6A2

Kat-Nr. : 66982-1-Ig

Synonyme

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

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Galerie der Validierungsdaten

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  WB analysis using 66982-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66982-1-Ig

  WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells.

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  WB analysis of A549 using 66982-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 66982-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, A549-Zellen, HepG2-Zellen, L02-Zellen, NCI-H1299-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66982-1-Ig bindet in WB, ELISA EPHX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPHX1 fusion protein Ag25820
• Vollständiger Name: epoxide hydrolase 1, microsomal (xenobiotic)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003567
• Gene symbol: EPHX1
• Gene ID (NCBI): 2052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).