ENTPD8 Polyklonaler Antikörper

ENTPD8 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21010-1-AP

Synonyme

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

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Galerie der Validierungsdaten

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  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 21010-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubierte HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21010-1-AP bindet in WB, ELISA ENTPD8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENTPD8 fusion protein Ag15224
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 8
• Berechnetes Molekulargewicht: 495 aa, 54 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 54 kDa
• GenBank-Zugangsnummer: BC141810
• Gene symbol: ENTPD8
• Gene ID (NCBI): 377841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.