Endoglin/CD105 Polyklonaler Antikörper

Endoglin/CD105 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 28117-1-AP

Synonyme

CD105, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

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Galerie der Validierungsdaten

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  WB analysis using 28117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Nierenzellkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

28117-1-AP bindet in WB, IHC, ELISA Endoglin/CD105 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Ag27822
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: ENG
• Gene ID (NCBI): 2022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publikationen

Species	Application	Title
human	IHC	Mol Cancer Res; A TGFβ-dependent stromal subset underlies immune checkpoint inhibitor efficacy in DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer.; Authors - Eisei Endo
human	IHC	Sci Rep; Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells; Authors - Kazuma Yoshida