ENDOG Polyklonaler Antikörper

ENDOG Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 22148-1-AP

Synonyme

Endo G, ENDOG, endonuclease G, Endonuclease G, mitochondrial

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Galerie der Validierungsdaten

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  WB analysis using 22148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 22148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 22148-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse kidney using 22148-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22148-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22148-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 22148-1-AP

  IP Result of anti-ENDOG (IP:22148-1-AP, 4ug; Detection:22148-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

22148-1-AP bindet in WB, IP, IHC, IF, ELISA ENDOG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENDOG fusion protein Ag17739
• Vollständiger Name: endonuclease G
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC016351
• Gene symbol: ENDOG
• Gene ID (NCBI): 2021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

Publikationen

Species	Application	Title
human	WB	Chem Biol Interact; O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.; Authors - Han Liao
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
human	WB	Front Pharmacol; Quercitrin Attenuates Acetaminophen-Induced Acute Liver Injury by Maintaining Mitochondrial Complex I Activity.; Authors - Weichen Xiong
human	WB	Front Pharmacol; Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.; Authors - Nian Yang
rat	WB	Metallomics; Induction of mitochondrial apoptosis pathway mediated through caspase-8 and c-Jun N-terminal kinase by cadmium-activated Fas in rat cortical neurons.; Authors - Shuangquan Wen
human	WB	Int J Mol Sci; Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells.; Authors - Jen-Jie Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (06-11-2021)

SDS-PAGE: 40 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mitochondrial fraction from mice brain tissue

ENDOG Antibody Western Blot validation (1:1000 dilution) in Mitochondrial fraction from mice brain tissue (Cat no:22148-1-AP)