ELK4 Polyklonaler Antikörper

ELK4 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14666-1-AP

Synonyme

ELK4, SAP 1, SAP1, SRF accessory protein 1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14666-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14666-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14666-1-AP bindet in WB, ELISA ELK4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELK4 fusion protein Ag6273
• Vollständiger Name: ELK4, ETS-domain protein (SRF accessory protein 1)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC063676
• Gene symbol: ELK4
• Gene ID (NCBI): 2005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELK4, also named as SAP1, SAP-1 and SAP1, belongs to the ETS family. It forms a ternary complex with the serum response factor (SRF). ELK4 requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. It is androgen regulated, involved in promoting cell growth, and highly expressed in a subset of prostate cancer. (PMID:19293179 )

Publikationen

Species	Application	Title
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
mouse	WB	J Pers Med; Exosomes from Microvascular Endothelial Cells under Mechanical Unloading Inhibit Osteogenic Differentiation via miR-92b-3p/ELK4 Axis; Authors - Xiaoyan Zhang