ELK1 Polyklonaler Antikörper

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• KD/KO Validated

ELK1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 27420-1-AP

Synonyme

ELK , ELK1

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Galerie der Validierungsdaten

• 

  WB analysis using 27420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27420-1-AP

  WB result of ELK1 antibody (27420-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELK1 transfected HepG2 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

27420-1-AP bindet in WB, IHC, IF, ChIP, ELISA ELK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELK1 fusion protein Ag26683
• Vollständiger Name: ELK1, member of ETS oncogene family
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC056150
• Gene symbol: ELK1
• Gene ID (NCBI): 2002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
rat	IHC	J Transl Med; The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy.; Authors - Xue Li
human	WB	Biochem Biophys Res Commun; CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.; Authors - Ranran Sun
human	WB	Genes Genomics; Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.; Authors - Akhlaq Ahmad ; KD Validated
human	WB	Cancer Res Commun; Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer; Authors - Liwei Lang ; KD Validated