ELAVL2 Monoklonaler Antikörper

ELAVL2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2D8C10

Rezensionen (1)

Kat-Nr. : 67097-1-Ig

Synonyme

ELAV like neuronal protein 1, ELAV like protein 2, ELAV2, ELAVL2, HEL N1, HELN1, Hu antigen B, HUB

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Galerie der Validierungsdaten

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  WB analysis using 67097-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67097-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67097-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67097-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 67097-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67097-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Maushirngewebe, Neuro-2a-Zellen, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

67097-1-Ig bindet in WB, IF, ELISA ELAVL2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ELAVL2 fusion protein Ag28310
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC030692
• Gene symbol: ELAVL2
• Gene ID (NCBI): 1993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publikationen

Species	Application	Title
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
mouse	WB	Sci Adv; The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity; Authors - Dominika Grzejda

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Petr (Verified Customer) (07-19-2023)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000