ELA2 Polyklonaler Antikörper

ELA2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 27642-1-AP

Synonyme

Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase

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Galerie der Validierungsdaten

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  WB analysis using 27642-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 27642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

27642-1-AP bindet in WB, IHC, IF, ELISA ELA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELA2 fusion protein Ag26634
• Vollständiger Name: elastase 2, neutrophil
• Berechnetes Molekulargewicht: 267 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC074816
• Gene symbol: ELA2
• Gene ID (NCBI): 1991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

Publikationen

Species	Application	Title
mouse	WB,IHC	Cell Mol Biol Lett; Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses.; Authors - Jin Li
bovine	IF	Animals (Basel); Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows.; Authors - Guanxin Lv
mouse	WB	Cancer Immunol Res; Tumor cell-derived microparticles induced by methotrexate augment T-cell antitumor responses by down-regulating expression of PD-1 in neutrophils; Authors - Pingwei Xu
rat	IHC	Front Pharmacol; RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis; Authors - Jiaqi Yao