EIF4E3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF4E3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17282-1-AP

Synonyme

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17282-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17282-1-AP

  Immunofluorescent analysis of Hela cells, using EIF4E3 antibody 17282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

17282-1-AP bindet in WB, IP, IHC, IF, ELISA EIF4E3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4E3 fusion protein Ag11056
• Vollständiger Name: eukaryotic translation initiation factor 4E family member 3
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC031289
• Gene symbol: EIF4E3
• Gene ID (NCBI): 317649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918). EIF4E3 exists two isoforms with MV 24 kDa and 13 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 30382096; Authors - Nourhan Abdelfattah ; KD Validated
human	WB, IP	Nat Commun; MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL.; Authors - Ari L Landon
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
human	WB	iScience; Transcriptome, proteome, and protein synthesis within the intracellular cytomatrix; Authors - Tattym E Shaiken
human	WB	Oncotarget; Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming.; Authors - Irene Riz ; KD Validated