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  • Featured Product
  • KD/KO Validated

EIF4E Monoklonaler Antikörper

EIF4E Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3C6B9

Kat-Nr. : CL594-66655

Synonyme

CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated EIF4E Monoclonal antibody (CL594-66655)

    IF Staining of HepG2 using CL594-66655

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66655 (EIF4E antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated EIF4E Monoclonal antibody (CL594-66655)

    FC experiment of HepG2 using CL594-66655

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EIF4E (CL594-66655, Clone:3C6B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66655 bindet in IF, FC (Intra) EIF4E und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EIF4E fusion protein Ag27191
Vollständiger Name eukaryotic translation initiation factor 4E
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC012611
Gene symbol EIF4E
Gene ID (NCBI) 1977
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 EIF4E antibody CL594-66655Protokoll herunterladen
FC protocol for CL594 EIF4E antibody CL594-66655Protokoll herunterladen
Standard-Protokolle
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