EIF3A Monoklonaler Antikörper

EIF3A Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1C7B4

Kat-Nr. : 67713-1-Ig

Synonyme

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

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Galerie der Validierungsdaten

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  WB analysis using 67713-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67713-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67713-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67713-1-Ig (EIF3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67713-1-Ig (EIF3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 67713-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using EIF3A antibody (67713-1-Ig, Clone: 1C7B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67713-1-Ig bindet in WB, IHC, IF, ELISA EIF3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF3A fusion protein Ag24762
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit A
• Berechnetes Molekulargewicht: 1382 aa, 166 kDa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: BC114429
• Gene symbol: EIF3A
• Gene ID (NCBI): 8661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Biomaterials; Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery; Authors - Jing-Si Jiang
human	WB,IF	Cell Prolif; G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration; Authors - Shuai Li
human	WB	Cell Death Differ; Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression; Authors - Yanan Pi