EIF2C1-Specific Polyklonaler Antikörper

EIF2C1-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 19690-1-AP

Synonyme

AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, EIF2C1-Specific, GERP95, hAgo1, Protein argonaute 1, Q99

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19690-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19690-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19690-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

19690-1-AP bindet in WB, IHC, IF, ELISA EIF2C1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2C, 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: NM_012199
• Gene symbol: EIF2C1
• Gene ID (NCBI): 26523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.

Publikationen

Species	Application	Title
human	IF	Front Mol Neurosci; miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.; Authors - Belinda J Goldie
human	WB	Mol Ther Oncolytics; Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis.; Authors - Xueting Wang
mouse	IF	bioRxiv; MicroRNAs are necessary for the emergence of Purkinje cell identity; Authors - Norjin Zolboot