EIF2AK2, PKR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF2AK2, PKR Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Rezensionen (1)

Kat-Nr. : 18244-1-AP

Synonyme

eIF 2A protein kinase 2, EIF2AK2, EIF2AK2, PKR, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

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Galerie der Validierungsdaten

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  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18244-1-AP

  WB result of EIF2AK2, PKR antibody (18244-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK2, PKR transfected HepG2 cells.

• 

  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18244-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18244-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 18244-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18244-1-AP

  IP Result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18244-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe, MCF-7-Zellen, Rattenherzgewebe, SH-SY5Y-Zellen, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

18244-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA EIF2AK2, PKR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Berechnetes Molekulargewicht: 551 aa, 62 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC093676
• Gene symbol: EIF2AK2
• Gene ID (NCBI): 5610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
mouse	WB	Nat Neurosci; Maternal immune activation in mice disrupts proteostasis in the fetal brain.; Authors - Brian T Kalish
human	WB	Sci Adv; Temporal control of the integrated stress response by a stochastic molecular switch.; Authors - Philipp Klein
human	WB	Nat Commun; NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.; Authors - Psylvia Léger
human	WB	J Immunother Cancer; Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment; Authors - Weiwei Lin
	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart