EIF2AK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF2AK1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 20499-1-AP

Synonyme

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

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Galerie der Validierungsdaten

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  WB analysis using 20499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20499-1-AP

  WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 20499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20499-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

20499-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA EIF2AK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF2AK1 fusion protein Ag14236
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 1
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC006524
• Gene symbol: EIF2AK1
• Gene ID (NCBI): 27102
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Publikationen

Species	Application	Title
human	WB,CoIP	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
human	WB	Science; Human NLRP1 is a sensor for double-stranded RNA.; Authors - Stefan Bauernfried
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KD Validated
human	WB	Mol Cell; Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.; Authors - Jack Chun-Chieh Hsu
human	WB,IP	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu