EHF Monoklonaler Antikörper

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• KD/KO Validated

EHF Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C12E4

Kat-Nr. : 67125-1-Ig

Synonyme

EHF, ESE 3, ESE3, ESE3B, ESEJ, ets homologous factor, hEHF

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Galerie der Validierungsdaten

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  WB analysis of LNCaP using 67125-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67125-1-Ig (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67125-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67125-1-Ig (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 67125-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67125-1-Ig (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67125-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67125-1-Ig (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 67125-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 67125-1-Ig (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A431-Zellen, humanes Hirngewebe, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67125-1-Ig bindet in WB, IHC, ELISA EHF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EHF fusion protein Ag28769
• Vollständiger Name: ets homologous factor
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC038995
• Gene symbol: EHF
• Gene ID (NCBI): 26298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Am J Cancer Res; Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.; Authors - Junqiang Li ; KD Validated