EHD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EHD1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 24657-1-AP

Synonyme

EH domain containing 1, EH domain containing protein 1, EHD1, H PAST, HPAST1, PAST, PAST homolog 1, PAST1, Testilin

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Galerie der Validierungsdaten

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  WB analysis using 24657-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24657-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 24657-1-AP

  IP result of anti-EHD1 (IP:24657-1-AP, 4ug; Detection:24657-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HeLa-Zellen, Maushirngewebe, Maushodengewebe, Rattenlungengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24657-1-AP bindet in WB, IP, IHC, IF, ELISA EHD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EHD1 fusion protein Ag18400
• Vollständiger Name: EH-domain containing 1
• Berechnetes Molekulargewicht: 534 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC104799
• Gene symbol: EHD1
• Gene ID (NCBI): 10938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	WB	Sci Rep; Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells.; Authors - Shaarvari Bhat ; KD Validated
human	WB,IHC,IF	BMC Cancer; PMID: 27411790; Authors - Jing Gao ; KD Validated
human	WB,IF	Biochem Biophys Res Commun; A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis.; Authors - Hiroki Yukimoto

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on thrombosis study. I use this for mouse platelet Western blot and it works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse platelet

FH

Juan (Verified Customer) (05-02-2019)

It works well with a single band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000-1:2000
• Cell Tissue Type: Hela cells