PHD3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PHD3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 18325-1-AP

Synonyme

Egl nine homolog 3, EGLN3, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

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Galerie der Validierungsdaten

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  WB analysis using 18325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18325-1-AP (PHD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 18325-1-AP

  WB result of PHD3 antibody (18325-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHD3 transfected HEK-293T cells.

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  IP experiment of A375 using 18325-1-AP

  IP Result of anti-PHD3 (IP:18325-1-AP, 4ug; Detection:18325-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18325-1-AP (PHD3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Produktinformation

18325-1-AP bindet in WB, IP, IHC, ELISA PHD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHD3 fusion protein Ag13197
• Vollständiger Name: egl nine homolog 3 (C. elegans)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-32 kDa
• GenBank-Zugangsnummer: BC010992
• Gene symbol: EGLN3
• Gene ID (NCBI): 112399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	IHC	Cancer Res; Prolyl Hydroxylase 3 Attenuates MCL-1-Mediated ATP Production to Suppress the Metastatic Potential of Colorectal Cancer Cells.; Authors - Praveenkumar Radhakrishnan
human	WB	Mol Ther; WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.; Authors - Tianli Chen ; KD Validated
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang
human	WB	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng
human	IHC	Med Oncol; Overexpression of the HIF hydroxylase PHD3 is a favorable prognosticator for gastric cancer.; Authors - Su Changlei C