EEF1G Monoklonaler Antikörper

EEF1G Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7C6

Kat-Nr. : 68148-1-Ig

Synonyme

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

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Galerie der Validierungsdaten

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  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68148-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 68148-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, Hausschwein-Herzgewebe, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Kaninchenherzgewebe, Kaninchenhirngewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68148-1-Ig bindet in WB, IF, ELISA EEF1G und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1G fusion protein Ag0218
• Vollständiger Name: eukaryotic translation elongation factor 1 gamma
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006520
• Gene symbol: EEF1G
• Gene ID (NCBI): 1937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.