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EEA1 Monoklonaler Antikörper

EEA1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E11E4

Kat-Nr. : CL488-68065

Synonyme

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EEA1 Monoclonal anti (CL488-68065)

    IF Staining of HeLa using CL488-68065

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EEA1 antibody (CL488-68065, Clone: 1E11E4 ) at dilution of 1:200, CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated EEA1 Monoclonal anti (CL488-68065)

    IF Staining of HepG2 using CL488-68065

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 EEA1 antibody (CL488-68065, Clone: 1E11E4 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated EEA1 Monoclonal anti (CL488-68065)

    FC experiment of HeLa using CL488-68065

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EEA1 (CL488-68065, Clone:1E11E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68065 bindet in IF, FC (Intra) EEA1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EEA1 fusion protein Ag28814
Vollständiger Name early endosome antigen 1
Berechnetes Molekulargewicht 162 kDa
Beobachtetes Molekulargewicht 162 kDa
GenBank-ZugangsnummerNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 EEA1 antibody CL488-68065Protokoll herunterladen
FC protocol for CL Plus 488 EEA1 antibody CL488-68065Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen