Endothelin 1 Monoklonaler Antikörper

Endothelin 1 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2C5B3

Kat-Nr. : 67008-1-Ig

Synonyme

EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1

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Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 67008-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67008-1-Ig (EDN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293T using 67008-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Endothelin 1 (67008-1-Ig, Clone:2C5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67008-1-Ig bindet in WB, FC, ELISA Endothelin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Endothelin 1 fusion protein Ag28334
• Vollständiger Name: endothelin 1
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC009720
• Gene symbol: EDN1
• Gene ID (NCBI): 1906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Publikationen

Species	Application	Title
human	WB	Biomed Rep; Cilostazol regulates the expressions of endothelin-1 and endothelial nitric oxide synthase via activation of the p38 MAPK signaling pathway in HUVECs; Authors - Ying Xue
human	WB	Hypertens Res; Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation; Authors - Zheng Wang