EBP2 Polyklonaler Antikörper

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• KD/KO Validated

EBP2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15973-1-AP

Synonyme

EBNA1 binding protein 2, EBNA1BP2, EBP2, NOBP, Nucleolar protein p40, P40

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 15973-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15973-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15973-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15973-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15973-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

15973-1-AP bindet in WB, ELISA EBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EBP2 fusion protein Ag8760
• Vollständiger Name: EBNA1 binding protein 2
• Berechnetes Molekulargewicht: 306 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC009175
• Gene symbol: EBNA1BP2
• Gene ID (NCBI): 10969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Oncol; EBP2, a novel NPM-ALK-interacting protein in the nucleolus, contributes to the proliferation of ALCL cells by regulating tumor suppressor p53.; Authors - Yuki Uchihara ; KD Validated