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Destrin Monoklonaler Antikörper

Destrin Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A12D9

Kat-Nr. : CL488-67657

Synonyme

ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated Destrin Monoclonal a (CL488-67657)

    FC experiment of MCF-7 using CL488-67657

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Destrin (CL488-67657, Clone:1A12D9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67657 bindet in FC (Intra) Destrin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Destrin fusion protein Ag1402
Vollständiger Name destrin (actin depolymerizing factor)
Berechnetes Molekulargewicht 19 kDa
Beobachtetes Molekulargewicht18-19 kDa
GenBank-ZugangsnummerBC009477
Gene symbol DSTN
Gene ID (NCBI) 11034
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 Destrin antibody CL488-67657Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen