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Desmin Polyklonaler Antikörper

Desmin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-16520

Synonyme

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    FC experiment of C2C12 using CL594-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Desmin (CL594-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMausherzgewebe
Erfolgreiche Detektion in FCC2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-16520 bindet in IF, FC (Intra) Desmin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Desmin fusion protein Ag9742
Vollständiger Name desmin
Berechnetes Molekulargewicht 470 aa, 54 kDa
Beobachtetes Molekulargewicht 52-53 kDa
GenBank-ZugangsnummerBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 Desmin antibody CL594-16520Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen