DYX1C1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DYX1C1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14522-1-AP

Synonyme

DYX1, DYX1C1, DYXC1, EKN1, RD

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Galerie der Validierungsdaten

• 

  WB analysis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14522-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14522-1-AP

  IP result of anti-DYX1C1(IP:14522-1-AP, 4ug; Detection:14522-1-AP 1:800) with mouse testis tissue lysate 1120 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

14522-1-AP bindet in WB, IP, IHC, IF, ELISA DYX1C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYX1C1 fusion protein Ag5997
• Vollständiger Name: dyslexia susceptibility 1 candidate 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC062564
• Gene symbol: DYX1C1
• Gene ID (NCBI): 161582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYX1C1 initially was described as the first candidate gene for developmental dyslexia. The protein contains three TPR motifs and one p23 domain, all commonly known for their protein-protein interactions. It also has been linked to neuronal migration. Recently it has been reported that the expression of DYX1C1 protein is lost in breast cancer and patients who have lost DYX1C1 expression have a poor overall survival,indicating that DYX1C1 is a potential prognostic biomarker in breast cancer.

Publikationen

Species	Application	Title
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A ; KO Validated
human	IF	Biol Psychiatry; Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins.; Authors - Kristiina Tammimies
human	WB,IF	Mol Endocrinol; The rs3743205 SNP is important for the regulation of the dyslexia candidate gene DYX1C1 by estrogen receptor β and DNA methylation.; Authors - Tammimies Kristiina K
human	IHC	BMC Cancer; The dyslexia candidate gene DYX1C1 is a potential marker of poor survival in breast cancer.; Authors - Rosin Gustaf G
human	WB,IP	BMC Mol Cell Biol; Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2; Authors - Andrea Bieder